Characterization of a Gelatin/Chitosan/Hyaluronan scaffold-polymer

• Javier Enrione ^[1] ; Fernando Osorio ^[1] ; Daniel López ^[1] ; Caroline Weinstein-Oppenheimer ^[2] ; Miguel A Fuentes ^[2] ; Ricardo Ceriani ^[2] ; Donald I Brown ^[2] ; Fernando Albornoz ^[3] ; Elizabeth Sánchez ^[3] ; Patricio Villalobos ^[3] ; Rodrigo A Somoza ^[3] ; Manuel E Young ^[3] ; Cristian A Acevedo ^[3]
  1. [1] Universidad de Santiago de Chile

     Universidad de Santiago de Chile

     Santiago, Chile

     
  2. [2] Universidad de Valparaíso

     Universidad de Valparaíso

     Valparaíso, Chile

     
  3. [3] Universidad Técnica Federico Santa María Centro de Biotecnología

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• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 13, Nº. 5, 2010, págs. 20-21
• Idioma: inglés
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• Resumen

  • Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30ºC could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties.