Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut

• Chuan Tang Wang ^[1] ; Shan Lin Yu ^[1] ; Shu Wei Zhang ^[1] ; Xiu Zhen Wang ^[1] ; Yue Yi Tang ^[1] ; Jian Cheng Zhang ^[1] ; Dian Xu Chen ^[1]
  1. [1] Shandong Peanut Research Institute
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 13, Nº. 5, 2010, págs. 18-19
• Idioma: inglés
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• Resumen

  • A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.