Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe

• Swaminathan Detchanamurthy ^[3] ; Kirubanandan Shanmugam ^[3] ; Salai Madhumathi Arasi Parkunan ^[3] ; Shilpa Puttananjaiah ^[1] ; Balaji Somasundaram ^[2] ; Kannan Subramanian ^[2]
  1. [1] University of Mysore

     University of Mysore

     India

     
  2. [2] University of Canterbury

     University of Canterbury

     Nueva Zelanda

     
  3. [3] Sri Venkateswara College of Engineering Department of Biotechnology Bioprocess and Downstream Processing Laboratory

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• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 13, Nº. 4, 2010, págs. 1-2
• Idioma: inglés
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  • S-Adenosyl Methionine (SAMe) Synthetase is an enzyme which catalyses the synthesis of S-Adenosyl Methionine using methionine and ATP. It is also known as AdoMet which is well known methyl donor, which modifies DNA, RNA, histones and other proteins, dictating replicational, transcriptional and translational fidelity, mismatch repair, chromatin modeling, epigenetic modifications and imprinting. The objective of the present work is to clone the SAMe Synthetase gene in recombinant E. coli strain in order to express, characterize and purify it for further synthesis of SAMe in a large scale fermentation. Expression was induced by 1 mM IPTG and expressed protein was characterized by SDS-PAGE. The recombinant E. coli cells were used for the production of SAMe through batch and fed batch fermentation operations. The produced SAMe was purified through paper chromatography in order to use it in our future studies.