Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)

• Yue Yi Tang ^[1] ; Chuan Tang Wang ^[3] ; Guan Pin Yang ^[1] ; Tong Feng ^[2] ; Hua Yuan Gao ^[2] ; Xiu Zhen Wang ^[3] ; Xiao Yuan Chi ^[3] ; Ya Long Xu ^[4] ; Qi Wu ^[3] ; Dian Xu Chen ^[3]
  1. [1] Ocean University of China

     Ocean University of China

     China

     
  2. [2] Jilin Academy of Agricultural Sciences

     Jilin Academy of Agricultural Sciences

     China

     
  3. [3] Shandong Peanut Research Institute
  4. [4] Zhengzhou Tobacco Research Institute of CNTC China Tobacco Gene Research Center

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• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 5, 2011, págs. 5-5
• Idioma: inglés
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• Resumen

  • To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).