Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L.)

• Shi-hua Shan ^[2] ; Ting-ting Zhang ^[2] ; Chun-juan Li ^[2] ; Chen Yang ^[2] ; Cai-xia Yan ^[2] ; Shu-bo Wan ^[1]
  1. [1] Shandong Academy of Agricultural Sciences

     Shandong Academy of Agricultural Sciences

     China

     
  2. [2] Shandong Peanut Research Institute
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 6, 2011, págs. 6-6
• Idioma: inglés
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• Resumen

  • Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.