EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)

• Guo Qi Song ^[1] ; Meng Jun Li ^[1] ; Han Xiao ^[1] ; Xing Jun Wang ^[1] ; Rong Hua Tang ^[2] ; Han Xia ^[1] ; Chuan Zhi Zhao ^[1] ; Yu Ping Bi ^[1]
  1. [1] Ministry of Agriculture Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province
  2. [2] Guangxi Academy of Agricultural Sciences Cash Crops Research Institute
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 13, Nº. 3, 2010, págs. 7-8
• Idioma: inglés
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• Resumen

  • Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA (9.0%), CTT/GAA (7.4%), and AAG/TTC (7.3%) repeats. Ninety-four SSR containing ESTs were randomly selected for primer design and synthesis, of which 33 pairs could generate good amplification and were used for polymorphism assessment. Results showed that polymorphism was very low in cultivars, while high level of polymorphism was revealed in wild type peanuts.