Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content

Chuan Tang Wang ^[2] ; Yue Yi Tang ^[2] ; Xiu Zhen Wang ^[2] ; Shu Wei Zhang ^[1] ; Gui Jie Li ^[2] ; Jian Cheng Zhang ^[2] ; Shan Lin Yu ^[2]
1. [1] Shandong Agricultural University
  
  Shandong Agricultural University
  
  China
2. [2] Shandong Peanut Research Institute
Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 2, 2011, págs. 10-10
Idioma: inglés
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Resumen
- Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.