High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses

• Magorzata Krzywonos ^[2] ; Thomas Eberhard ^[1]
  1. [1] Swedish University of Agricultural Sciences

     Swedish University of Agricultural Sciences

     Uppsala domkyrkoförs., Suecia

     
  2. [2] Wroc?aw University of Economics Department of Bioprocess Engineering
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 2, 2011, págs. 6-6
• Idioma: inglés
• Enlaces
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• Resumen

  • Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10% molasses (v/v), with NH4OH used for pH adjustment, may be used as a media for large-scale cultivation of L. plantarum. Such composition of the medium permits a high density of lactic acid bacteria (1.6 x 10(10) cfu/ml) to be achieved. Conclusions: The use of a fermentation medium consisting of distillery wastewater and molasses to obtain value-added products (such as LAB biomass and lactic acid) is a possible step for classical ethanol production to move towards a biorefinery model production in which all by and waste products are utilized to increase produced values and reduce waste production. This enables a cost-effective utilization of the problematic wastewater from ethanol and sugar production.