A preferable approach to clone hLIF cDNA from the genomic DNA

• Yihong Cui ^[1] ; Guangqin Zhu ^[1] ; Qiuju Chen ^[1] ; Yunfei Wang ^[1] ; Mingming Yang ^[1] ; Yuxuan Song ^[1] ; Jiangang Wang ^[1] ; Binyun Cao ^[1]
  1. [1] Northwest Agriculture and Forestry University College of Animal Science and Technology
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 3, 2011, págs. 12-12
• Idioma: inglés
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• Resumen

  • Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.