Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
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Álvaro Sequeida [2] ; Eduardo Tapia [2] ; Marcelo Ortega [1] ; Pablo Zamora [3] ; Álvaro Castro [1] ; Christian Montes [4] ; Gustavo E Zúñiga [1] ; Humberto Prieto [4]
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[1] Universidad de Santiago de Chile
Universidad de Santiago de Chile
Santiago, Chile
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[2] Universidad Técnica Federico Santa María-Pontificia Universidad Católica de Valparaíso Programa Doctorado en Biotecnología
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[3] University of California-Davis Plant Sciences Department
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[4] Instituto de Investigaciones Agropecuarias Centro Regional de Investigación La Platina
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- Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 15, Nº. 4, 2012, págs. 8-8
- Idioma: inglés
- Enlaces
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Resumen
Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA.
