Callus induction and plant regeneration of Ulex europaeus

• Ingrid Ramírez ^[1] ; Fernando Dorta ^[1] ; Álvaro Cuadros-Inostroza ^[2] ; Hugo Peña-Cortés ^[1]
  1. [1] Universidad Técnica Federico Santa María Centro de Biotecnología D. Alkalay L. Laboratorio de Biología Molecular
  2. [2] Max Planck Institute of Molecular Plant Physiology
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 15, Nº. 4, 2012, págs. 7-7
• Idioma: inglés
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• Resumen

  • A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.