Optimization of β-carotene production by a newly isolated Serratia marcescens strain

Bohua Wang ^[3] ; Liping Lin ^[1] ; Lei Lu ^[2] ; Weiping Chen ^[1]
1. [1] Jiangxi Agricultural University
  
  Jiangxi Agricultural University
  
  China
2. [2] Northeast Forestry University
  
  Northeast Forestry University
  
  China
3. [3] Hunan University of Arts and Science Zoology Key Laboratory of Hunan Higher Education
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Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 15, Nº. 6, 2012, págs. 3-3
Idioma: inglés
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Resumen
- β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 µg/mL.