Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum

• Mauricio Díaz ^[1] ; Victor Polanco ^[2] ; Ingrid Ramírez ^[3] ; Hugo Peña-Cortés ^[3]
  1. [1] Pontificia Universidad Católica de Valparaíso

     Pontificia Universidad Católica de Valparaíso

     Valparaíso, Chile

     
  2. [2] Universidad Mayor Instituto de Biotecnología Centro de Genómica y Bioinformática
  3. [3] Universidad Técnica Federico Santa María Centro de Biotecnología Daniel Alkalay Lowitt

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• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 15, Nº. 1, 2012, págs. 10-10
• Idioma: inglés
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• Resumen

  • Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene.