Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
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Weiping Yang [1] ; Fanxu Meng [1] ; Jiayin Peng [1] ; Peng Han [1] ; Fang Fang [1] ; Li Ma [1] ; Binyun Cao [1]
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[1] Northwest A & F University College of Animal Science and Technology
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- Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 17, Nº. 6, 2014, págs. 262-267
- Idioma: inglés
- Enlaces
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Resumen
Background The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig. Results A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8-12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24-48 h. Conclusions The isolated BY-2 strain rapidly grew and produced cellulase. We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics.
