Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino

• JAIME LÓPEZ M ^[1] ; CARMEN GLORIA ILI G ^[1] ; PRISCILLA BREBI M ^[1] ; PATRICIA GARCÍA M ^[1] ; ITALO CAPURRO V ^[1] ; PABLO GUZMÁN G ^[1] ; EUGENIO SUÁREZ P ^[2] ; JOSÉ MANUEL OJEDA F ^[3] ; JUAN CARLOS ROA S ^[1]
  1. [1] Universidad de La Frontera

     Universidad de La Frontera

     Temuco, Chile

     
  2. [2] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     
  3. [3] Universidad Diego Portales

     Universidad Diego Portales

     Santiago, Chile

     

  Mostrar afiliaciones +
• Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 138, Nº. 11, 2010, págs. 1343-1350
• Idioma: español
• Títulos paralelos:
  • Human papillomavirus genotyping of cervical uterine preneoplastic lesions in a high risk area
• Enlaces
  • Texto completo
• Resumen

  • The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. Aim: To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. Material and Methods: Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. Results: HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. Conclusions: HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.