Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile
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JORGE LÉVICAN [1] ; MAURICIO TORRES [1] ; NICOLÁS GAGGERO [1] ; ROSA CORVALÁN [1] ; ALDO GAGGERO [1]
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[1] Universidad de Chile
Universidad de Chile
Santiago, Chile
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- Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 139, Nº. 2, 2011, págs. 143-149
- Idioma: español
- Títulos paralelos:
- Parvovirus B19 among blood donors from three hospitals in Santiago, Chile
- Enlaces
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Resumen
Background: Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions. Aim: To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases. Material and Methods: A total of477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR. Results: Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5,626 x 10(5) genome equivalents/ml. Conclusions: Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.
