Encapsulation of leu-enkephalin in core-shell isobutylcyanoacrylate - thiolated chitosan nanoparticles for oral administration

• VÍCTOR H CAMPOS REQUENA ^[1] ; KAWTHAR BOUCHEMAL ^[1] ; CHRISTINE VAUTHIER ^[1] ; GILLES PONCHEL ^[1]
  1. [1] UniversityParis-South XI Faculty of Pharmacy
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 53, Nº. 4, 2008, págs. 1677-1681
• Idioma: inglés
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  • The present study shows the evaluation of Leu-Enkephalin (Leu-Enk) encapsulation on core-shell isobutylcyanoacrylate/thiolated chitosan (IBCA/ChitoTBA) nanoparticles as a tool of combined system of polymers and colloidal particles for oral administration of poor absorption drugs. Leu-Enk encapsulation using anionic emulsión polymerization technique was carried out previous depolymerization of MW 400,000 gmol"¹ commercial chitosan, followed by thiolation of this depolymerized chitosan with Traut's reagent (2-iminothiolane), and final characterization. Later, direct quantification of the encapsulated Leu-Enk into the nanoparticles was carried out evaluating two methodologies for nanoparticles rupture solubilization with: NaOH 1 N and DMSO. Physico-chemical characterization of the obtained nanoparticles showed diameters in the nano-range between 28 and 46 nm, and yields of encapsulation of Leu-Enk between 25 and 46%, and recovery yields over 96% for co-polymer nanoparticles solubilization method with DMSO.