Development and validation of a gc-npd/micro-ecd method using dual column for the determination of benzodiazepine in human whole blood and plasma

• FELIPE BRAVO ^[1] ; CLAUDIO LOBOS ^[1] ; KARINA VENEGAS ^[2] ; JULIO BENITES ^[1]
  1. [1] Universidad Arturo Prat

     Universidad Arturo Prat

     Iquique, Chile

     
  2. [2] Servicio Médico Legal Laboratorio Referencial Norte
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 55, Nº. 4, 2010, págs. 454-457
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Four benzodiazepines flunitrazepam, nitrazepam, clonazepam, and alprazolam, have been analyzed from biological matrices by dual column gas chromatography using a nitrogen-phosphorus detector (NPD) and a micro-electron capture detector (m-ECD). The recoveries of the four benzodiazepines spiked into human whole blood and plasma were 88.8 - 97.9 and 90.7 - 97.5%, respectively. The regression equations for the four benzodiazepines showed excellent linearity. The detection limits (LODs) were 0.14 - 0.95 ng/mL for whole blood and 0.13 - 0.93 ng/mL for plasma. The method is simple and sensitive for the determination of benzodiazepines in human whole blood and plasma, and may be useful in forensic science practice.