Hplc determination of etoposide in injectable dosage forms

• M MUNAWAR HAYAT ^[1] ; MUHAMMAD ASHRAF ^[1] ; NISAR UR-REHMAN ^[2] ; FAIZ UL-HASSAN NASIM ^[1] ; IRSHAD AHMAD ^[1] ; JAMEEL RAHMAN ^[1] ; MUHAMMAD SALEEM ^[1] ; M ZUBAIR MALIK ^[1]
  1. [1] Islamia University of Bahawalpur

     Islamia University of Bahawalpur

     Pakistán

     
  2. [2] COMSATS Institute of Information Technology

     COMSATS Institute of Information Technology

     Pakistán

     
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 56, Nº. 4, 2011, págs. 881-883
• Idioma: inglés
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• Resumen

  • 1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form.