Biosensors' preparation to be used for entrapment method with copolymers polyaniline derivatives

• C. O SANCHEZ ^[1] ; A ISLA ^[1] ; C BUSTOS ^[1] ; F DÍAZ ^[3] ; N GATICA ^[2]
  1. [1] Universidad Austral de Chile

     Universidad Austral de Chile

     Valdivia, Chile

     
  2. [2] Universidad de Concepción

     Universidad de Concepción

     Comuna de Concepción, Chile

     
  3. [3] Universidad Católica de Chile Facultad de Química Departamento de Química Orgánica

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• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 55, Nº. 2, 2010, págs. 233-235
• Idioma: inglés
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  • The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at pH 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H2O2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of currentresponse, limitedbythe cyclenumbers of the cyclic voltammetry, 232 s, andenough stability to allow for the measurements to be taken after several days.