In vitro characterization of antioxidant properties of cuban endemic varieties of erythroxylum alaternifolium a. Rich. Isolation of two flavonol glycosides

• WILMER H PERERA CÓRDOVA ^[1] ; MISLÉN GÓMEZ MATOS ^[1] ; JESSICA TABART ^[2] ; ARNAUD SIPEL ^[2] ; CLAIRE KEVERS ^[2] ; JACQUES DOMMES ^[2]
  1. [1] Instituto de Ecologia y Sistemática

     Instituto de Ecologia y Sistemática

     Cuba

     
  2. [2] University of Liège

     University of Liège

     Arrondissement de Liège, Bélgica

     
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 57, Nº. 4, 2012, págs. 1340-1343
• Idioma: inglés
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• Resumen

  • The total antioxidant capacity from leaves of three Cuban endemic varieties of Erythroxylum alaternifolium was measured using three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and Oxygen radical absorbance capacity (ORAC). The highest values of antioxidant capacity and total phenols were measured in polar n-butyl alcohol extracts. Among the varieties of E. alaternifolium assayed, the var. parvifolium showed the strongest antioxidant capacity: ABTS (6.49 ± 0.05 mg TE/g dw); DPPH (11.16 ± 0.01 mg TE/g dw) and ORAC (35.1 ± 1.5 mg TE/g dw). Additionally, n-butyl alcohol extract of E. alaternifolium var. parvifolium also showed the highest content in phenolics with 284.2 ± 7.3 mg CAE/g dw and in flavonols with skeleton quercetin, kaempferol and myricetin with 471 μg/g dw. Moreover, two flavonol glycosides: ombuin-3-O-rutinoside and quercetin-3-O-rutinoside were isolated for the first time from n-butyl alcohol extract of E. alaternifolium var. alaternifolium.