Covalent immobilization of glucose oxidase on well-defined polymer-silicon wafer hybrids via surface-initiated raft-mediated process

• JIAYOU JI ^[1] ; KEQIANG XIA ^[1] ; TINGTING LIU ^[1] ; LIANG LI ^[1]
  1. [1] Wuhan Institute of Technology

     Wuhan Institute of Technology

     China

     
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 58, Nº. 3, 2013, págs. 1872-1875
• Idioma: inglés
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• Resumen

  • A surface modification technique was developed for the functionalization of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with glycidyl methacrylate (GMA) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. GOD was then covalently immobilized through the ring-opening reaction between the amine groups of the GOD and the epoxide groups of the grafted GMA polymer chains. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified surface after each modification stage. Increasing the thickness of the polymer layer and the immobilization time could allow a great amount of GOD to be immobilized on the silicon surface. The GOD-functionalized silicon hybrids are promising candidates for the silicon-based glucose biosensors.