Colombia
El objetivo de este trabajo fue aplicar la metodología de criopreservación de semen canino en pajilla francesa en el municipio de Medellín- Antioquia. Se realizó una colecta seminal a ocho caninos adultos, por método de mano enguantada, cada eyaculado fue evaluado macroscópica y microscópicamente y posteriormente diluido con Triladyl® a una concentración final de 80 x 106 espermatozoides/ml. Se empacaron en pajillas de 0,5 ml y fueron sometidas a criopreservación con un descenso de temperatura rápido, sometiendo las pajillas a vapores de nitrógeno durante 25 minutos y luego transferidas directamente a nitrógeno líquido (método de pajilla francesa). En el semen fresco, se observó una apariencia blanquecina lechosa, un volumen de 2.27 ml (±1.40) una concentración de 388.5 x106 espermatozoides/ mL (±228.069), una motilidad individual de 79% (± 4%) y un vigor de 3.96 (±0.327). Se obtuvieron 123 pajillas, de las cuales se tomaron 27 aleatoriamente y se les evaluó la motilidad individual (51 ± 19%) y vigor (2.89 ± 1.02). En conclusión, la aplicación de la metodología de criopreservación rápida por pajilla francesa, usando Triladyl® como crioprotector, se sugiere como protocolo eficiente para la criopreservación de semen canino en el municipio de Medellín.
The objective of this work was to apply the canine semen cryopreservation methodology in French straw in Medellín-Antioquia. A seminal collection was carried out to eight adult canines, by gloved hand method, each ejaculate was assessed macroscopically and microscopically and subsequently diluted with Triladyl® with a final concentration of 80x106 sperm/ml. Semen samples were packed in 0.5 ml straws and a quick-freezing process was performed (nitrogen vapors for 25 minutes and then transferred directly to liquid nitrogen, french straw method). In fresh semen, a milky whitish appearance, a volume of 2.27 ml (± 1.40), a concentration of 388.5x106 sperm/mL (± 228.069), individual motility of 79% (± 4%) and vigor of 3.96 (± 0.327) were observed. 123 straws were obtained, of which 27 were taken randomly and individual motility (51 ± 19%) and vigor (2.89 ± 1.02) were evaluated. In conclusion, the application of the methodology of quick-freezing by french straw, using Triladyl® as a cryoprotectant, is suggested as an efficient protocol for cryopreservation of canine semen in Medellín-Antioquia.
The objective of this work was to apply the canine semen cryopreservation methodology in French straw in Medellín-Antioquia. A seminal collection was carried out to eight adult canines, by gloved hand method, each ejaculate was assessed macroscopically and microscopically and subsequently diluted with Triladyl® with a final concentration of 80x106 sperm/ml. Semen samples were packed in 0.5 ml straws and a quick-freezing process was performed (nitrogen vapors for 25 minutes and then transferred directly to liquid nitrogen, french straw method). In fresh semen, a milky whitish appearance, a volume of 2.27 ml (± 1.40), a concentration of 388.5x106 sperm/mL (± 228.069), individual motility of 79% (± 4%) and vigor of 3.96 (± 0.327) were observed. 123 straws were obtained, of which 27 were taken randomly and individual motility (51 ± 19%) and vigor (2.89 ± 1.02) were evaluated. In conclusion, the application of the methodology of quick-freezing by french straw, using Triladyl® as a cryoprotectant, is suggested as an efficient protocol for cryopreservation of canine semen in Medellín-Antioquia.
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