Iodate oxidation of n-acetyl l-cysteine: Application in drug determination and characterization of its oxidation and degradation product by mass spectrometry

MASOOM RAZA SIDDIQUI ^[1] ; SAIKH MOHAMMAD WABAIDUR ^[1] ; ZIED A ALOTHMAN ^[1] ; HABIBUR RAHMAN ^[3] ; SARFARAZ ALAM ^[2] ; SAJID ALI ^[2]
1. [1] King Saud University
  
  King Saud University
  
  Arabia Saudí
2. [2] Jazan University
  
  Jazan University
  
  Arabia Saudí
3. [3] Aligarh Muslim University Chemistry Department
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Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 59, Nº. 1, 2014, págs. 2303-2307
Idioma: inglés
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Resumen
- A kinetic spectrophotometric method based on the initial rate measurement has been developed for the determination of N-acetyl L-cysteine. The developed method is based on the oxidation of N-acetyl L-cysteine with iodate. The reaction product was studied and characterized using the mass spectrometry and the structure of the product was proposed. From the mass spectrometric studies it was concluded that the oxidation of the drug resulted in the formation of a disulfide. The developed method was validated as per the guidelines of international conference on harmonization. The developed initial rate method was found to be linear in the concentration range of 1.25 - 30 µg ml-1. The detection and quantitation limits were found to be 0.018 and 0.056 µg ml-1. In the current study, the degradation product of N-acetyl L cysteine was also prepared and identified using mass spectrometry.