Validation of a method to detect central nervous system acting drugs in blood by gc/ms. Application in cases of death caused by phenothiazines in the north of chile

Felipe Bravo; Roberto Ulloa; Karina Venegas; Maria Cecilia Contzen; David Ríos; Margarita Briceño; Pedro Buc Calderoni; Julio Benites

Ayuda

Validation of a method to detect central nervous system acting drugs in blood by gc/ms. Application in cases of death caused by phenothiazines in the north of chile

FELIPE BRAVO ^[1] ; ROBERTO ULLOA ^[1] ; KARINA VENEGAS ^[2] ; MARIA CECILIA CONTZEN ^[2] ; DAVID RIOS ^[1] ; MARGARITA BRICEÑO ^[1] ; PEDRO BUC CALDERONI ^[1] ; JULIO BENITES ^[1]
1. [1] Universidad Arturo Prat
  
  Universidad Arturo Prat
  
  Iquique, Chile
2. [2] Servicio Médico Legal Laboratorio Referencial Norte
Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 59, Nº. 3, 2014, págs. 2603-2605
Idioma: inglés
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Resumen
- A simple and rapid analytical method is developed and validated for the determination of antiepileptic and antipsychotic drugs in human whole blood using a solid-phase extraction and quantification by gas chromatography-mass spectrometry (GC/MS). Prazepam was used as internal standard (IS). The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection and quantification were in the range of 0.52 - 0.98 μg/mL and 1.56 - 2.97 μg/mL for antiepileptic drugs (pentobarbital, phenobarbital and carbamazepine) and 1.31 - 2.94 ng/mL and 3.98 - 8.89 ng/mL for antipsychotic drugs (chlorpromazine and thioridazine), respectively. The relative standard deviation (RSD) was less than 7.07%, while the intra-day accuracy was < 3.21% and the inter-day was < 8.80%, referred to RSD. The developed methodology was applied in forensic cases of death caused by phenothiazines in the North of Chile during 2010-2012.