Impaired intratumoral dendritic cell function and potential predictive value of dendritic cell markers for metastasis in malignant salivary gland tumors

• Ana Guadalupe Gama-Cuellar ^[1] ; Katya Pulido Díaz ^[2] ; Mariana Martínez Calleja ^[3] ; Gabriela Anaya Saavedra ^[3] ; Velia Ramírez-Amador ^[3] ; Jorge Rodarte Corro ^[4] ; Victor Ramón-Ramírez ^[4] ; Ricardo Luiz Albuquerque-Júnior ^[1] ; Rogério Gondak ^[1]
  1. [1] Universidade Federal de Santa Catarina

     Universidade Federal de Santa Catarina

     Brasil

     
  2. [2] Universidad Autónoma de Baja California

     Universidad Autónoma de Baja California

     México

     
  3. [3] DDS, MSc, PhD. Autonomous Metropolitan University, Xochimilco Unit, Department of Health Care, Mexico City, State of Mexico, Mexico
  4. [4] MD. Mexican Institute of Social Security, Regional General Hospital No.1, Tijuana, Baja California, Mexico

  Mostrar afiliaciones +
• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 29, Nº. 2 (March), 2024
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The differentiation between primary and metastatic salivary gland neoplasms (SGNs) helps in determining appropriate management strategies, including the need for additional diagnostic tests, surveillance, or aggressive treatment. The purpose of this study was to identify and quantify the immature and mature dendritic cells (DCs) in metastatic and no metastatic SGNs and determine its association with clinicopathological findings.

    Cross-sectional, observational, and descriptive study that includes 33 malignant salivary gland neoplasms [MSGN (6, 18.1% metastatic)], and 22 pleomorphic adenomas (PA), as a control group. Clinical and histopathological characteristics were obtained. Immunohistochemistry for human leukocyte antigen D-related (HLA-DR), CD1a, CD83, and Ki-67 proteins was done. Positive intra- and peritumoral DCs were counted.

    Individuals with MSGN had a lower density of intratumoral HLA-DR+ cells than those with PA (p=0.001), Ki-67 immunostaining was significantly higher in MSGN than in PA (6% vs. 1.4%, p<0.001). Metastatic MSGN showed less intratumoral CD1a+ than non-metastatic (3.2 vs. 165.1, p=0.001). No differences in intra- and peritumoral CD83+ cells were found between benign and malignant SGN.

    These results suggest that the immune-protective function of intratumoral DCs is compromised in MSGNs. DCs markers may represent useful prediction tools for metastases in salivary gland malignancies, with crucial implications in the implementation of appropriate disease management strategies.