Confirmation of deoxyniv alenol presence in chilean wheat by high-performance thin-layer chromatography-mass spectrometry

• MARIO VEGA-HERRERA ^[1] ; RICARDO MADARIAGA ^[2] ; MARIO ARANDA ^[1] ; GERTRUD E MORLOCK ^[3]
  1. [1] Universidad de Concepción

     Universidad de Concepción

     Comuna de Concepción, Chile

     
  2. [2] INIA-Quilamapu
  3. [3] Justus Liebig University Giessen Institute of Nutritional Science

  Mostrar afiliaciones +
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 62, Nº. 2, 2017, págs. 3435-3437
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Deoxynivalenol (DON) is a mycotoxin that belongs to trichothecene subgroup B. Due its detrimental effects for human health, DON has been analyzed and detected by nearly all chromatographic methods. The present work reports for the first time the application of HPTLC/MS to confirm the presence of DON in wheat crops. Chromatography was performed on silica gel 60 F254 HPTLC plates using toluene - ethyl acetate - formic acid 6:3:1 (V/V/V) as mobile phase. After post-chromatographic derivatization with 10 % AlCl3, fluorescence detection was carried out at UV 366/>400 nm. Bands identity and purity was confirmed by mass spectrometry acquiring analytes directly from the sample bands by means of TLC/MS elution head-based interface. Both, sample and standard bands showed clear mass signals at m/z 341 that corresponds to the formate adduct of DON [M+HCOO]-. Thus, the presence of DON in the Chilean wheat sample was confirmed.