Early gut microbiological changes and metabolomic changes in patients with sepsis: a preliminary study

• Yang Zhou ^[1] ; Yinghao Luo ^[1] ; Xibo Wang ^[1] ; Feiyu Luan ^[1] ; Yahui Peng ^[1] ; Yue Li ^[2] ; Xiaohui Ma ^[1] ; Xiaonan Jia ^[1] ; Nana Li ^[1] ; Mingyin Man ^[1] ; Jieling Wei ^[1] ; Yuanyuan Ji ^[1] ; Qianqian Zhang ^[1] ; Chunying Wang ^[1] ; Wenjing Mu ^[3] ; Jun Wang ^[1] ; Changsong Wang ^[1] ; Mingyan Zhao ^[1] ; Kaijiang Yu ^[1]
  1. [1] First Affiliated Hospital of Harbin Medical University

     First Affiliated Hospital of Harbin Medical University

     China

     
  2. [2] Second Affiliated Hospital of Harbin Medical University

     Second Affiliated Hospital of Harbin Medical University

     China

     
  3. [3] Harbin Medical University

     Harbin Medical University

     China

     

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• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 26, Nº. 4, 2023, págs. 1131-1142
• Idioma: inglés
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• Resumen

  • The gut microbiota is closely related to the development of sepsis. The aim of this study was to explore changes in the gut microbiota and gut metabolism, as well as potential relationships between the gut microbiota and environmental factors in the early stages of sepsis. Fecal samples were collected from 10 septic patients on the first and third days following diagnosis in this study. The results showed that in the early stages of sepsis, the gut microbiota is dominated by microorganisms that are tightly associated with inflammation, such as Escherichia-Shigella, Enterococcus, Enterobacteriaceae, and Streptococcus. On sepsis day 3 compared to day 1, there was a significant decrease in Lactobacillus and Bacteroides and a significant increase in Enterobacteriaceae, Streptococcus, and Parabacteroides. Culturomica_massiliensis, Prevotella_7 spp., Prevotellaceae, and Pediococcus showed significant differences in abundance on sepsis day 1, but not on sepsis day 3. Additionally, 2-keto-isovaleric acid 1 and 4-hydroxy-6-methyl-2-pyrone metabolites significantly increased on sepsis day 3 compared to day 1. Prevotella_7 spp. was positively correlated with phosphate and negatively correlated with 2-keto-isovaleric acid 1 and 3-hydroxypropionic acid 1, while Prevotella_9 spp. was positively correlated with sequential organ failure assessment score, procalcitonin and intensive care unit stay time. In conclusion, the gut microbiota and metabolites are altered during sepsis, with some beneficial microorganisms decreasing and some pathogenic microorganisms increasing. Furthermore, Prevotellaceae members may play different roles in the intestinal tract, with Prevotella_7 spp. potentially possessing beneficial health properties and Prevotella_9 spp. potentially playing a promoting role in sepsis.