Comparing the effect of benzalkonium chloride-preserved, polyquad-preserved, and preservative-free prostaglandin analogue eye drops on cultured human conjunctival goblet cells

• Anne Hedengran ^{[1] [2]} ; Josefine Freiberg ^[1] ; Pernille May Hansen ^{[1] [2]} ; Gerard Boix-Lemonche ^{[3] [4]} ; Tor P. Utheim ^{[3] [3]} ; Darlene A. Dartt ^[5] ; Goran Petrovski ^[3] ; Steffen Heegaard ^{[2] [2]} ; Miriam Kolko ^{[1] [2]}
  1. [1] University of Copenhagen

     University of Copenhagen

     Dinamarca

     
  2. [2] Copenhagen University Hospital

     Copenhagen University Hospital

     Dinamarca

     
  3. [3] Oslo University Hospital

     Oslo University Hospital

     Noruega

     
  4. [4] University of Oslo

     University of Oslo

     Noruega

     
  5. [5] Harvard Medical School

     Harvard Medical School

     City of Boston, Estados Unidos

     

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• Localización: Journal of Optometry: peer-reviewed Journal of the Spanish General Council of Optometry, ISSN-e 1888-4296, Vol. 17, Nº. 1, 2024
• Idioma: inglés
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  • Purpose To investigate the effect of benzalkonium chloride (BAK)-preserved latanoprost and bimatoprost, polyquad (PQ)-preserved travoprost, and preservative-free (PF) latanoprost and tafluprost, all prostaglandin analogues (PGAs), on human conjunctival goblet cell (GC) survival. Furthermore, to investigate the effect of BAK-preserved and PF latanoprost on the cytokine secretion from GC.

    Methods Primary human conjunctival GCs were cultivated from donor tissue. Lactate dehydrogenase (LDH) and tetrazolium dye colorimetric (MTT) assays were used for the assessment of GC survival. A cytometric bead array was employed for measuring secretion of interleukin (IL)-6 and IL-8 from GC.

    Results BAK-preserved latanoprost and bimatoprost reduced cell survival by 28% (p = 0.0133) and 20% (p = 0.0208), respectively, in the LDH assay compared to a negative control. BAK-preserved latanoprost reduced cell proliferation by 54% (p = 0.003), BAK-preserved bimatoprost by 45% (p = 0.006), PQ-preserved travoprost by 16% (p = 0.0041), and PF latanoprost by 19% (p = 0.0001), in the MTT assay compared to a negative control. Only PF tafluprost did not affect the GCs in either assay. BAK-preserved latanoprost caused an increase in the secretion of pro-inflammatory IL-6 and IL-8 (p = 0.0001 and p = 0.0019, respectively) compared to a negative control, which PF latanoprost did not.

    Conclusion BAK-preserved PGA eye drops were more cytotoxic to GCs than PQ-preserved and PF PGA eye drops. BAK-preserved latanoprost induced an inflammatory response in GC. Treatment with PF and PQ-preserved PGA eye drops could mean better tolerability and adherence in glaucoma patients compared to treatment with BAK-preserved PGA eye drops.