Variation of butyrate production in the gut microbiome in type 2 diabetes patients

• Julienne Siptroth ^[2] ; Olga Moskalenko ^[3] ; Carsten Krumbiegel ^[3] ; Jörg Ackermann ^[1] ; Ina Koch ^[1] ; Heike Pospisil ^[2]
  1. [1] Goethe University Frankfurt

     Goethe University Frankfurt

     Frankfurt, Alemania

     
  2. [2] Technical University of Applied Sciences Wildau
  3. [3] BIOMES NGS GmbH

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• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 26, Nº. 3, 2023, págs. 601-610
• Idioma: inglés
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  • Background Diabetes mellitus type 2 is a common disease that poses a challenge to the healthcare system. The disease is very often diagnosed late. A better understanding of the relationship between the gut microbiome and type 2 diabetes can support early detection and form an approach for therapies. Microbiome analysis offers a potential opportunity to find markers for this disease. Next-generation sequencing methods can be used to identify the bacteria present in the stool sample and to generate a microbiome profile through an analysis pipeline. Statistical analysis, e.g., using Student’s t-test, allows the identification of significant differences. The investigations are not only focused on single bacteria, but on the determination of a comprehensive profile. Also, the consideration of the functional microbiome is included in the analyses. The dataset is not from a clinical survey, but very extensive.

    Results By examining 946 microbiome profiles of diabetes mellitus type 2 sufferers (272) and healthy control persons (674), a large number of significant genera (25) are revealed. It is possible to identify a large profile for type 2 diabetes disease. Furthermore, it is shown that the diversity of bacteria per taxonomic level in the group of persons with diabetes mellitus type 2 is significantly reduced compared to a healthy control group. In addition, six pathways are determined to be significant for type 2 diabetes describing the fermentation to butyrate. These parameters tend to have high potential for disease detection.

    Conclusions With this investigation of the gut microbiome of persons with diabetes type 2 disease, we present significant bacteria and pathways characteristic of this disease.