Natalia Houghton, Marlén Martínez Gutierrez, Dioselina Peláez, Jaime E. Castellanos
Objetivo. Realizar el seguimiento de la infección in vitro por enterovirus 71 (EV71) sobre células TE-671, evaluando la presencia de los antígenos virales y la pérdida de viabilidad en diferentes tiempos de infección. Materiales y métodos. Se infectaron cultivos de células TE-671 con diferentes diluciones de EV71, aislado de un caso de parálisis flácida aguda (3364-COL-94), y por varios tiempos posinfección (6, 12, 24, 48, 72 horas). Los cultivos se procesaron por una técnica de inmunoperoxidasa indirecta para obtener las proporciones de infección en cada condición. Simultáneamente, se realizó una prueba de viabilidad por el método bioquímico del MTT para evaluar la pérdida de la viabilidad celular debida a la infección.
Objective. To study in vitro infection of TE-671 cells by Enterovirus 71 (EV71), determining viral antigens and cell mortality due to infection at different infection times. Materials and methods. TE-671 cells were infected with dilutions of a clinical isolate of EV71 (3364-COL-94) at various postinfection (pi) times (6, 12, 24, 48, 72 hours). Cell cultures were processed by an indirect immunoperoxidase technique to find cell infection percentages for each condition. Simultaneously, infected and non-infected cell cultures were processed by MTT viability test, to evaluate cytopathic effect due to the virus. Results. Despite viral antigens were initially detected at 6 hours pi, only after 48 hours pi cellular mortality and cytopathic effect were most evident. Viral antigens were detected with low cell mortality at 12 hours pi. Conclusion. The high susceptibility of TE-671 to EV71 infection was confirmed, which correlates well with strong cytopathic effect induced by EV71 infection. This cell line is a useful tool for viral isolation and identification of this virus, as well as for basicresearch.
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