Genetic Diversity of Cephalopods (Mollusca: Cephalopoda) from Bangladesh Waters Based on COI and 16S rRNA Genes
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Noor Aida Arfin [1] ; Anindita Barua [1] ; Sujan Kumar Datta [1] ; Sumaiya Ahmed [2] ; Md. Sagir Ahmed [1]
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[1] University of Dhaka
University of Dhaka
DCC (Kotwali), Bangladés
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[2] Jagannath University
Jagannath University
DCC (Kotwali), Bangladés
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- Localización: Thalassas: An international journal of marine sciences, ISSN 0212-5919, Vol. 39, Nº. 2 (October 2023), 2023, págs. 1199-1205
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
In this study, we aim to determine the efficacy of mitochondrial cytochrome c oxidase I (COI) and 16S ribosomal RNA genes as marker in discriminating the cephalopods from Bangladesh waters. We generated 30 DNA sequences from 28 newly collected specimens, representing eight species belonging to seven genera and three families. The species were discriminated with threshold of ≥ 3% genetic distance between species for both marker genes. The mean divergence between species was 40 and 15-fold higher than the average conspecific distance for the COI and 16S rRNA, respectively. The Neighbor-Joining (NJ) tree topology showed that the sequences of the same species were clustered in a single clade, supporting the efficacy of marker genes in differentiating cephalopod species and further complementing the conventional taxonomy. The study confirms the efficacy of both COI and 16S rRNA as candidate markers for discriminating cephalopods species. However, we recommend the use of COI, as it showed less intra-species divergence and less amplification and sequencing failure compared to 16S rRNA.
