Resumen de Frecuencia de aislamientos ambientales de Staphylococcus aureus y su actividad beta-lactamasa en un hospital de Cajamarca, Perú

Marco Rivera Jacinto, Claudia Rodríguez Ulloa, Gladys Huayán Dávila

• español

  Objetivo. Determinar la frecuencia de aislamientosde Staphylococcus aureus y su capacidadpara producir beta-lactamasas clásicas,en reservorios ambientales de las salas deoperaciones del Servicio de Cirugía y de lassalas de partos del Servicio de Obstetricia delHospital Regional de Cajamarca, Perú.Metodología. Las muestras analizadaspara el presente estudio fueron obtenidasmediante hisopados de las superficies del mobiliarioy de otros objetos inanimados que seencontraban en las salas. Para el aislamientoe identificación se empleó agar selectivo manitolsalado, coloración Gram y las pruebas decatalasa y coagulasa. Para la determinaciónde beta-lactamasas clásicas, se empleó el métodoyodométrico.

• English

  Objetive: To determine the frequency of Staphylococcus aureus isolates, and its capacity to produce classic beta-lactamases, in environmental fomites in operations rooms and delivery rooms at the Hospital Regional de Cajamarca, Perú.Methods: Analyzed samples for the present study were obtained by cottons swabs from furniture surfaces and other inanimate objects that were in the rooms. Mannitol salt agar, Gram coloration, catalase and coagulase tests were used for starin isolation and identification. The iodometric method was used for the determination of classic beta-lactamases.Results: Out of 64 samples, 17 isolates were obtained and identified as S. aureus, 3 of which were producing classic beta-lactamases and were found in fomites from stretchers and tables.Conclusions: This study showed a moderate frequency of S. aureus with capacity to produce beta-lactamases, potentially multiresistant, in environmental fomites at the hospital.