In this work, the effects of two micronutrients (Mn2+, H2Bo3-) and macronutrients (PO4(3-) and NO3-) from biofertilizeres resources on growth (leaf dry/fresh weight, root dry/fresh weight, stem/root length, number of stem) and accumulation of essential oils in Lemon balm (Melissa officinalis L.) were investigated. The most of essential oils in two seasons were Caryophyllene oxide, E-Caryophyllene, Geranial, Geraniol, Chavicol and Neral that affected by the treatments. Of the two micronutrients, manganese, and in macronutrients NO3- were more effective in stimulating the accumulation of components. At 150 ppm of micronutrients by biofertilizeres, enhanced the production of citronellal, Chavicol. Although combination of H2Bo3-and Mn2+ at 300 ppm by macronutrients in some of essential oils like neral, caryophyllene oxide and 14-hydroxy-Z-caryophyllene were more produced than 150 ppm combinations but most of essential oils significantly increased in 150 ppm concentration of micronutrients with macronutrients. Exo-Isocitral, Chavicol, 14-hydroxy-Z-Caryophyllene and Germacrene D, were upper extracted in 150 ppm concentration of micronutrients but in many combinations this essential oil was no extracted in little concentration.
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