Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.

• V Chandrakar ^[1] ; A Dubey ^[2] ; S Keshavkant ^[1]
  1. [1] Ravishankar Shukla University School of Studies in Biotechnology
  2. [2] Chhattisgarh Council of Science and Technology Central Laboratory Facility
• Localización: Journal of soil science and plant nutrition, ISSN-e 0718-9516, ISSN 0718-9508, Vol. 16, Nº. 3, 2016, págs. 662-676
• Idioma: inglés
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• Resumen

  • To investigate the physiological and metabolic attributes of arsenic (As) stress tolerance conferred by exogenous salicylic acid (SA), Glycine max L. (variety JS 335) seeds were aseptically germinated over filter paper moistened with SA (500 µM) and/or10 and 100 µM As (Sodium arsenite was used). On 2nd and 5th days of germination, the growing radicles were harvested, and analyzed for growth and different metabolic attributes. Findings exemplified that As significantly decreased germination percentage, radicle length, dry mass and activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), while stimulated the contents of As, reactive oxygen species (ROS), lipoxygenase (LOX), guaiacol peroxidase (POD) and proline. Additionally, isozymes of antioxidants were also scrutinized over Native-PAGE gels and were found to be altered considerably under As-stress. However, exogenous SA remarkably enhanced germination percentage, growth indices, activities of SOD, CAT and APX, and proline accumulation along with reduced As, ROS and LOX, and restoring POD in As-stressed seedlings. In conclusion, SA confers As-stress tolerance to Glycine max L. by regulating the antioxidant enzymes and proline accumulation thereby reduced As content and ROS production. Further study is intended, particularly at gene level, to understand precise mechanism(s) involved in SA-mediated As-stress tolerance.