Histopathology and molecular identification of Henneguya pseudoplatystoma

Romário Oliveira de Sales; Juliana Rosa Carrijo Mauad; Claucia Aparecida Honorato; Kesia Esther da Silva; Jaqueline Verconti; Peceu Magyve Ragagnin; Sibele Borsuk; Maurício Laterça Martins; Simone Simionatto

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Histopathology and molecular identification of Henneguya pseudoplatystoma

Oliveira de Sales, Romário ^[1] ; Carrijo Mauad, Juliana Rosa ^[1] ; Honorato, Claucia Aparecida ^[1] ; da Silva, Kesia Esther ^[1] ; Verconti, Jaqueline ^[1] ; Ragagnin, Peceu Magyve ^[1] ; Borsuk, Sibele ^[2] ; Laterça Martins, Mauricio ^[3] ; Simionatto, Simone ^[1]
1. [1] Universidade Federal da Grande Dourados
  
  Universidade Federal da Grande Dourados
  
  Brasil
2. [2] Universidade Federal de Pelotas
  
  Universidade Federal de Pelotas
  
  Brasil
3. [3] Universidade Federal de Santa Catarina
  
  Universidade Federal de Santa Catarina
  
  Brasil
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Localización: Latin American Journal of Aquatic Research, ISSN-e 0718-560X, ISSN 0716-1069, Vol. 48, Nº. 2, 2020, págs. 207-213
Idioma: inglés
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Resumen
- The present study proposes to characterize the parasites isolated during the initial phase of production in fish farms located in Mato Grosso do Sul in the central-western region of Brazil, using histopathology analysis and molecular techniques. A total of 340 hybrid surubim fish (Pseudoplatystoma reticulatum × P. corruscans) from four farms were examined during the co-feeding phase. Histopathology analysis showed that 10.9% (n = 37) of the fish were infected with parasites. Branchitis, lifting epithelium, hypertrophy of epithelial cells, lamellar fusion, aneurisms and infection in the bone tissue of the gill filament was observed. The parasite species was determined by amplification of the 18S rRNA gene followed by sequencing. The phylogenetic analysis of nucleotide sequences indicates a close relationship (99.6%) with Henneguya pseudoplatystoma reported to be infecting the hybrid Pseudoplatystoma. This study demonstrates the occurrence of H. pseudoplatystoma in hybrid surubim (P. reticulatum × P. corruscans) during the co-feeding phase in fish farms in Mato Grosso do Sul. Also, molecular techniques provide a faster and sensitive method to identify fish parasites, and may assist in the development of new management techniques aimed at improving the sanitary conditions contributing to the reduction of mortality rates in these animals.