Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil

• Coelho-Mello, Maria Verônyca ^[1] ; Florindo-Guedes, Maria Izabel ^[1] ; Rodriguez-Málaga, Sergio ^[2] ; de Almeida, Lia Magalhães ^[1] ; de Freitas Moreira, Mariana ; de Oliveira, Tatiane Rodrigues ^[1]
  1. [1] Universidade Estadual do Ceará
  2. [2] Universidade Federal do Pará and Universidade Estadual do Ceará
• Localización: Latin American Journal of Aquatic Research, ISSN-e 0718-560X, ISSN 0716-1069, Vol. 42, Nº. 3, 2014, págs. 649-652
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The shrimp Litopenaeus vannamei, one of the most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes up to 70% mortalities. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolated IMNV, from L. vannamei farmed in Brazil, through a discontinuous sucrose gradient, and sequenced cDNA fragment encoding the major capsid protein from this virus. Nucleotides sequences corresponding to the viral capsid protein was obtained by RT-PCR and confirmed by automatic sequencing. Comparison with sequences which encode the capsid protein obtained from Indonesia isolates showed a high identity.