Resumen de Sustainable Alternative to Antimicrobial Uses: New Probiotics
Samuel Gómez Martínez, Apeh Omede, Manuel Gómez García, Héctor Puente Fernández, Lucía Pérez Pérez, Ana María Carvajal Urueña, Héctor Argüello Rodríguez
Indiscriminate use of antimicrobials is linked to a hazardous increase in antimicrobial resistances (AMR). Global concern in AMR has restricted antimicrobial use through new tight legislation and research on new sustainable alternatives is needed. Probiotics with their ability to positively regulate the growth of beneficial bacteria of the intestinal microbiota are proposed as a solution to this problem. The aim of this study was to isolate and characterise the probiotic potential of 44 lactic acid bacteria (LAB) obtained from sow’s colostrum and milk. Two candidates were selected after determining the AMR profile in this collection for further in vitro assays: a Lactiplantibacillus plantarum (LA-34 M) and a Loigolactobacillus coryniformis (LA-10). Their antibacterial activity was demonstrated against three different enteric pathogens (Escherichia coli, Salmonella spp., and Clostridium perfringens). Adherence test were performed using the epithelial cell line IPI-21, where La-10 and La-34 showed highly adherence to cell culture (64% and 68% respectively). Furthermore, an invasion assay against Salmonella Typhimurium demonstrated that booth strains were able to reduce significantly (p < 0.05) S. Typhimurium invasion after 1 and 2 h of incubation. These results show once more the interest of LAB in pathogen control. Further in vitro and in vivo assays will allow to determine the commercial potential of these two isolates.
