We present the first complete and commented list of the ichthyofauna of the Ría Deseado (47°45´S, 65°55´W) and record the southernmost limit distribution of five species. The systematic checklist comprises 44 species belonging to 4 classes, 15 orders, 26 families, and 35 genera. The largest Families are Nototheniidae (8) and Zoarcidae (6). The ichthyofauna is mainly composed of Magellanic fauna, while some Bonaerensean components are also present. Our results broaden the number of known fish species in the region, contributing as an input for the development of conservation and management plans for this area.
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