Nanosuspensions as carriers of Annona muricata acetogenins: antibacterial activity against Enterococcus faecalis and listeria monocytogenes
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Brandon Alexis López-Romero [1] ; Guillermo Téllez-Isaías [4] ; Gabriel Luna-Bárcenas [2] ; Adalberto Zamudio-Ojeda [3] ; Gilberto Velázquez-Juárez [3] ; María de Lourdes Garcia-Magaña [1] ; Gabriela Aguilar-Hernández [3] ; Efigenia Montalvo-González [1]
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[1] Tecnológico Nacional de México
Tecnológico Nacional de México
México
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[2] Instituto Tecnológico y de Estudios Superiores de Monterrey
Instituto Tecnológico y de Estudios Superiores de Monterrey
México
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[3] Universidad de Guadalajara
Universidad de Guadalajara
México
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[4] University of Arkansas
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- Localización: CyTA: Journal of food, ISSN 1947-6337, ISSN-e 1947-6345, Vol. 21, Nº. 1, 2023, págs. 771-780
- Idioma: inglés
- Enlaces
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Resumen
This research aimed to develop nanosuspensions as carriers of Annona muricata acetogenins (ACGs) using different concentrations of soy lecithin (SL), hydroxypropyl-β-cyclodextrin (βCD) and the dispersion method (low agitation, middle agitation and ultrasound) on particle size and polydispersity index (PDI). A response surface analysis was performed to find an optimized, validated and characterized nanosuspension (OβCDSL-ACGs-NSps) and to measure its antibacterial activity. The best conditions to formulate OβCDSL-ACGs-NSps with spherical nanoparticles (133.77 nm), PDI (0.20) and good stability (−37 mV) were 10 mg/mL SL, 0.16% βCD and ultrasound as a dispersion method. Nanoparticles from OβCDSL-ACGs-NSps exhibited a high entrapment (85.33%) and encapsulation efficiency (56.09%). The highest release (84.15%) of ACGs was observed until 36 h. OβCDSL-ACGs-NSps also showed significant antibacterial inhibition against E. faecalis (87%) and L. monocytogenes (75%) at 320 µg/mL. βCDSL-ACGs-NSps is an attractive and promising alternative to improve the solubility and bioavailability of ACGs in pharmaceutical applications.
