NCK1-AS1 promotes the proliferation, migration, invasion, and EMT of non-small cell lung cancer by regulating the miR-361-5p/ADAM10 axis

• Bingchen Wu ^[2] ; Zizong Wang ^[1] ; Hanlin Xu ^[3] ; Xiangyang Chu ^[1] ; Qiwen Jiang ^[2]
  1. [1] Chinese PLA General Hospital

     Chinese PLA General Hospital

     China

     
  2. [2] Hospital of Chinese Medicine of Changxing County, Huzhou, Zhejiang, China
  3. [3] the Affiliated Hospital of Qingdao, Qingdao, Shandong, China

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 12, 2023, págs. 1453-1464
• Idioma: inglés
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• Resumen

  • Lung cancer, one of the most frequently diagnosed cancers, causes a huge number of mortalities globally. Among lung cancers, non-small cell lung cancer (NSCLC) is the most recorded. Despite accumulating research, the molecular basis of NSCLC progression remains poorly known. Therefore, we aim to assess the function of NCK1-AS1 in NSCLC and elucidate the molecular mechanism. Firstly, we quantified the NCK1-AS1 level in tumors and adjacent healthy tissues. NCK1-AS1 was significantly upregulated in NSCLC tumors, which was associated with poor prognosis in patients. Silencing NCK1-AS1 significantly inhibited the proliferation, migration, and invasion, as well as the EMT of NSCLC cell lines.

    Starbase bioinformatic prediction revealed that NCK1- AS1 targets miR-361-5p which acts to regulate ADAM10 gene expression. Our result showed that NCK1-AS1 upregulation markedly reduced miR-361-5p mRNA expression, while increasing ADAM10 expression. For the first time, we demonstrated that NCK1-AS1 regulates the miR-361-5p/ADAM10 axis, thereby promoting NSCLC progression. NCK1-AS1 might be developed as a therapeutic target for treating NSCLC.