Heat stress induced in vitro affects cell viability and gene expression of dermal fibroblasts from bovine and buffalo

• BORGES, Natasha P. ^[2] ; SOUZA, Eduardo B. ^[1] ; SANTOS, Simone S. D. ^[1] ; OHASHI, Otávio M. ^[1] ; SANTANA, Priscila P. B. ^[3] ; SILVA-FILHO, Ednaldo ^[3]
  1. [1] Universidad Federal de Pará

     Universidad Federal de Pará

     Brasil

     
  2. [2] Post-graduate Programme of Health and Animal Production. Federal Rural University of Amazonia - UFRA, Belém, Pará, Brazil
  3. [3] Post-graduate Programme of Applied Biotechnology in Agrarian Science. Federal Rural University of Amazonia - UFRA, Belém, Pará, Brazil

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• Localización: Spanish journal of agricultural research, ISSN-e 2171-9292, ISSN 1695-971X, Vol. 21, Nº. 4, 2023
• Idioma: inglés
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• Resumen

  • Aim of study: To evaluate the response of dermal fibroblasts to heat stress and different time exposures on the cell survival and gene expression.

    Area of study: Belém city, Pará state. Brazil.

    Material and methods: Fibroblasts were isolated from ear skin of bovine (n= 4) and buffalo (n= 4), cultured in vitro until the 3rd passage and submitted to heat stress at 42°C for 3, 6 and 12 h, except for the negative control (38.5°C for 24 h). Cell survival was measured using Trypan blue, and RNA isolation was performed using Trizol method following qRT-PCR to quantify the relative expression of the inducible heat shock protein HSPA1A, the pro-apoptotic BAX and pro-inflammatory IFN-γ genes.

    Main results: Heat stress induced in vitro affected the cell viability and gene expression in a time-dependent manner. Gene expression was relatively lower in buffalo (p<0.05) than in bovine. Until 3 h of heat stress, HSPA1A showed a slight increase in both bovine and buffaloes, and BAX was 5.82-fold greater in bovine (p<0.05). After 6 h, HSPA1A was 75.81-fold (p<0.0001) and INF-γ was 20.15-fold greater (p<0.05) in bovine than buffalo. Only after 6 h the cell viability started to decrease significantly (p<0.05) in both species.

    Research highlights: Dermal fibroblasts of buffaloes and bovine were sensitive to heat stress induced in vitro, which was most detrimental to cell survival after 6 h. The expression of HSPA1A, BAX and INF-γ genes in response to heat stress indicate a slight sensibility of the dermal fibroblasts of bovine compared to their buffalo counterpart.