circRNA-SMO upregulates CEP85 to promote proliferation and migration of glioblastoma via sponging miR-326

• Bin Wu ^[2] ; Liang Xia ^[1] ; Shuyuan Zhang ^[1] ; Kai Jin ^[1] ; Caixing Sun ^[1] ; Liwen Li ^[1] ; Ting Xia ^[3] ; Gao Chen ^[2]
  1. [1] Zhejiang Cancer Hospital

     Zhejiang Cancer Hospital

     China

     
  2. [2] The Second Affiliated Hospital of Medical College of Zhejiang University, China
  3. [3] Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, China

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 11, 2023, págs. 1307-1319
• Idioma: inglés
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• Resumen

  • Circular RNAs (circRNAs) play an important role in cancer development by sponging microRNAs (miRNAs) to regulate the signaling axis. However, more comprehensive mechanisms of circRNAs in glioblastoma need to be elucidated.

    RT-qPCR was used to detect the expression levels of circRNA-SMO and miR-326. Dual-luciferase reporter assays were conducted to verify the interaction among circRNA-SMO, miR-326, and CEP85. Flow cytometric analysis was performed to detect apoptosis. Western blotting was used to determine the protein levels of the different molecules. Animal xenograft experiments were performed to evaluate the role of circRNA-SMO in vivo.

    CircRNA-SMO was upregulated in glioblastoma tissues and glioblastoma cells. CircRNA-SMO downregulation inhibited the viability and colonyforming ability of the glioblastoma cells. In addition, miR-326 was downregulated in glioblastoma cells, which was verified to sponge circRNA-SMO and interact with CEP85. Moreover, circRNA-SMO inhibition induced the elevation of miR-326 and apoptosis, accompanied by a decrease in CEP85.

    CircRNA-SMO knockdown-mediated tumor inhibition was prevented by an miR-326 inhibitor. Furthermore, circRNA-SMO inhibition inhibited tumor growth in vivo, accompanied by an increase in miR-326 and a decline in CEP85 in tumor tissues.

    Conclusions. CircRNA-SMO sponges miR-326 to promote glioblastoma proliferation and migration by upregulating CEP85 expression. This study clarified the role of circRNA-SMO in the development of glioblastoma, providing novel insights for its treatment.