Comprehensive analysis and identification of prognostic biomarkers and immunotherapeutic targets in the NADPH oxidase family (and its regulatory subunits) in pancreatic ductal adenocarcinoma

• Ruiqi Guo ^[1] ; Panchun Zheng ^[1] ; Shasha Zhu ^[2] ; Zhen Zeng ^[3] ; Zhenyu Li ^[3] ; Yaying Yang ^[1]
  1. [1] Chongqing Medical University

     Chongqing Medical University

     China

     
  2. [2] First Affiliated Hospital of Chongqing Medical University

     First Affiliated Hospital of Chongqing Medical University

     China

     
  3. [3] Department of Pathology, Chongqing University Cancer Hospital, Chongqing, China

  Mostrar afiliaciones +
• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 25, Nº. 12 (December), 2023, págs. 3460-3470
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Purpose This study aimed to evaluate the role of NADPH in pancreatic ductal adenocarcinoma using bioinformatic analyses and experimental validations.

    Methods We compared the expression levels, performed GO and KEGG analysis of NADPH oxidase family and its regulatory subunits, and determined the survival of patients with pancreatic ductal adenocarcinoma by GEPIA, David and KM plotter. The relationship between their expression with immune infiltration levels, phagocytotic/NK cell immune checkpoints, recruitment-related molecules were detected by Timer 2.0 and TISIDB, respectively. Subsequently, their correlation with NK cell infiltration level was verified by immunohistochemistry.

    Results The expression of some members of the NADPH oxidase family and its regulatory subunits was significantly increased in pancreatic ductal adenocarcinoma tissues compared to that in normal tissues and was positively correlated with natural killer (NK) cell infiltration. Furthermore, the NADPH oxidase family and its regulatory subunits were associated with survival and immune status in patients with pancreatic ductal adenocarcinoma, including chemokines, immune checkpoints, and immune infiltration levels of NK cells, monocytes, and myeloid-derived suppressor cells.

    Conclusions These results suggest the NADPH oxidase family and its regulatory subunits might serve as indicators for predicting the responsiveness to immunotherapy and outcome of patients with pancreatic ductal adenocarcinoma, providing a new perspective or strategy for immunotherapy in pancreatic ductal adenocarcinoma.