The partitioning of a dye, Brooker’s merocyanine (MOED), between water and 1-octanol and between water and dichloromethane is strikingly visible because of the dye’s strong solvatochromism, which makes the colors of the two layers different. The color change makes it easy to see that the dye has moved from the organic layer to the water layer or vice versa. A simple comparison of the color of the organic layer to reference solutions makes it possible to estimate rough values of KD, the distribution equilibrium constant.
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