Ex vivo detection of lipopolysaccharide immunopositivity in Rushton bodies

• Sirke Virkkunen ^[1] ; Jaana Willberg ^[2] ; Caj Haglund ^[1] ; Malin Sund ^[1] ; Timo Sorsa ^[3] ; Jaana Hagström ^[1]
  1. [1] University of Helsinki

     University of Helsinki

     Helsinki, Finlandia

     
  2. [2] University of Turku

     University of Turku

     Turku, Finlandia

     
  3. [3] Karolinska Institute

     Karolinska Institute

     Suecia

     

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 8, 2023, págs. 889-892
• Idioma: inglés
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• Resumen

  • Aim. Our aim was to investigate how bacterial lipopolysaccharide (LPS) is immunoexpressed in periapical lesions. By surprise we detected Rushton bodies (RBs) whose origin has been debatable to be positive for LPS.

    Methodology. Samples of radicular cysts (N=70) were stained in order to identify variations in LPS immunoexpression indicating bacterial background. For immunostaining, we used an anti-LPS antibody from Escherichia coli, and for visualization Horse Radish Peroxidase labeled polymer as the secondary antibody.

    Results. RBs showed positivity for LPS in radicular cysts. After collection of radicular cyst samples (70 in total), we noted that all RBs (N=25) histologically detected in tissue samples were positive for LPS.

    Furthermore, calcification in the cyst capsule showed immunopositivity.

    Conclusion. We demonstrate for the first time that LPS is present in RBs, indicating that host response to bacteria might be the initial cause of the formation of these hyaline bodies in the cyst epithelium and cyst capsule calcifications.