Manipulative-based Activity Using Pop Beads for Demonstration of Sanger Sequencing
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[1] Black Hills State University
Black Hills State University
City of Spearfish, Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 100, Nº 8, 2023, págs. 3138-3143
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Sanger sequencing, also known as dideoxy sequencing, is a widely used method for DNA sequencing, particularly for cloned plasmids and clinical samples. This technique requires a combination of essential biochemistry skills, such as a chain-termination reaction, gel electrophoresis, and fluorescence detection. Unfortunately, there is a lack of activities that replicate the Sanger sequencing process for students to learn and practice these skills. To address this issue, a manipulative-based Sanger sequencing activity was developed that incorporates colorful pop beads to demonstrate a chain-termination reaction, separation of products, and fluorescence detection. The beads represent deoxynucleotides and dideoxynucleotides, allowing for a visual representation of DNA fragment generation. This kinesthetic learning activity offers a high visual impact for students, aiding in their understanding of the Sanger sequencing process, and can also be used to illustrate polymerase chain reaction (PCR)-based techniques.
