Evaluation of Acid-Lugol’s Solution for Preservation of Acantharia (Radiolaria)

• Emiko Takano ^[1] ; Yasuhide Nakamura ^[2] ; Kazutaka Takahashi ^[1]
  1. [1] University of Tokyo

     University of Tokyo

     Japón

     
  2. [2] Shimane University

     Shimane University

     Japón

     
• Localización: Thalassas: An international journal of marine sciences, ISSN 0212-5919, Vol. 38, Nº. 2 (October 2022), 2022, págs. 939-945
• Idioma: inglés
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• Resumen

  • Acantharia are marine planktonic unicellular eukaryotes belonging to Radiolaria that are difficult to preserve in formalin seawater due to the rapid disintegration of their internal skeleton composed of strontium sulfate (SrSO4; also known as celestite) upon death. Adding Sr at concentrations 10–20 times higher than that in natural seawater to formalin is effective in preventing skeletal dissolution, although this method has not been applied to routine plankton sampling. In this study, we examined the effectiveness of 2% acid-Lugol’s solution for preserving Acantharia. The concentration of Acantharia in field-collected samples stored in a 250-mL bottle containing 2% acid-Lugol’s solution did not change significantly over 390 days. In contrast, the concentration of Acantharia stored in 5% formalin seawater with Sr addition significantly decreased over this period, indicating that acid-Lugol’s solution is a more effective preservative for the cells. These results expand the opportunities for studying Acantharia which have been considered as a difficult group to be quantified in routine microplankton sampling.