Hybrid Model Teaching in the Postepidemic Period: From Nucleic Acid to Antigen for the Fluorescence Analysis of SARS-CoV-2

• Wenxian Zhang ^[1] ; Ran Liu ^[1] ; Yang Shi ^[1] ; Hang Xing ^[2] ; Jingjing Zhang ^[1]
  1. [1] Nanjing University

     Nanjing University

     China

     
  2. [2] Hunan University

     Hunan University

     China

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 100, Nº 6, 2023, págs. 2339-2346
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Owing to the global spread of the coronavirus disease 2019 (COVID-19), education has shifted to distance online learning, whereas some face-to-face courses have been resumed with the improvement of the outbreak prevention and management situation, including a laboratory course for senior undergraduate students in chemical biology. Here, we present an innovative chemical biology experiment covering COVID-19 topics, which was created for third-year undergraduates. The basic principles of two nucleic-acid- and antigen-based diagnostic techniques for SARS-CoV-2 are demonstrated in detail. These experiments are designed to provide students with comprehensive knowledge of COVID-19 and related diagnoses in daily life. Crucially, the biosafety of this experimental manipulation was ensured by using artificial nucleic acids and recombinant protein. Furthermore, an interactive hybrid online-facing teaching model was designed to cover the key mechanism regarding PCR and serological tests of COVID-19. Finally, a satisfactory evaluation was obtained through a questionnaire, and simultaneously, reasonable improvements to the course design were suggested. The proposed curriculum provides all the necessary information for other instructors to create new courses supported by research.