Circ_0031242 regulates the functional properties of hepatocellular carcinoma cells through the miR-944/MAD2L1 axis

• Jianwei Lin ^[1] ; Zenghai Lin ^[1] ; Yaqiong Hua ^[2] ; Yan Chen ^[2]
  1. [1] First Affiliated Hospital of Shantou University Medical College

     First Affiliated Hospital of Shantou University Medical College

     China

     
  2. [2] Shenzhen Letu Biotechnology Co., Ltd., Guangdong, China
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 38, Nº. 3, 2023, págs. 303-316
• Idioma: inglés
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• Resumen

  • Background. Circular RNAs (circRNAs) possess key functions in the pathogenesis of hepatocellular carcinoma (HCC). Nonetheless, the actions of individual circRNAs in HCC remain undefined.

    Methods. circ_0031242, miR-944, and MAD2L1 expression were quantified by qRT-PCR. Transwell assay was utilized to examine cell invasion and migration. Glucose consumption and lactate production were measured to assess the impact on glycolysis. The relationships among circ_0031242, MAD2L1, and miR944 were examined via luciferase reporter assay.

    Results. circ_0031242 was notably augmented in HCC. Loss of function of circ_0031242 hindered cell proliferation, invasion, migration, glycolysis, and promoted apoptosis, as well as impeding HCC tumor growth. circ_0031242 directly targeted miR-944.

    Inhibition of miR-944 counteracted the effects of sicirc_0031242 on HCC cells. Additionally, miR-944 was proved to directly target MAD2L1 in HCC cells.

    Moreover, the promotion of MAD2L1 was able to rescue the inhibition of high miR-944 expression on HCC cell progression. Meanwhile, circ_0031242 involved the post-transcriptional modulation of MAD2L1 through miR-944.

    Conclusion. This study suggested that circ_0031242 regulated tumor cell progression and tumor growth through the miR-944/MAD2L1 axis in HCC.